The Discipher platform
Read-out mechanisms
Biochemical assays

Introducing a low cost generic diagnostic test platform, based on the hardware of an optical disc drive with:

Temperature control up to 80°C
Centrifugal microfluidics
Sensitivity to optical changes
Multiplexed samples / tests
Portability – plugs in to any computer
Pre-deposited reagents
Low volumes required
Real time analysis

By basing a generic diagnostics instrument on an optical drive, we are building on 30+ years of development, resulting in a robust, highly engineered and cost efficient product.

The Discipher platform’s rotational microfluidics disc simplifies sample handling and offers automation of process steps. As the discs are manufactured by standard methods and materials used in the optical disc industry for decades, the platform truly provides a low cost solution for a diverse range of diagnostic applications.
Functionalities that the disc offers are e.g.:

Simple injection of sample and additional solutions into disc
Sealing of inlet and outlet holes

The Discipher platform works by detecting optical changes in a biological sample, brought about via rotational microfluidics in combination with a number of well established techniques in the field of biological assays, such as:

Silver enhancement-can detect single enhancement processes – very sensitive
Colourimetric detection to < 0.5% absolute
Turbidity change
Submicron resolution imaging
Bead or cell imaging, counting

The types of assays that may be performed on this platform include:

Nucleic acid based assays (RNA/DNA)
Antibody assays
Cell based assays

Application areas
Possible areas of application for this platform include, but not limited to:

Diagnostics e.g. pathogen identification and host genotyping
Veterinary medicine
Wellness testing
Food allergies
Quality monitoring e.g. GMO detection, salmonella

LingVitae has IP rights to a patent portfolio covering optical disc based assays and biological testing. The work has been part-funded by the EU FP7 Digital Sequencing Programme, which uses a modified optical disc drive to perform DNA sequencing.